For this purpose, we exploit discontinuities in the commuter allowance scheme and employ a research design resting on a large panel of individual tax returns.
Seven days later, the reactivity of CD8+ splenocytes to a panel of individual peptides representing previously identified vaccinia virus CD8+ T cell epitopes [55], was determined by intracellular cytokine staining and flow cytometric analysis.
The PrPres profiles from brain and spleen were then compared by performing Western blot analyses of the brain and spleen in a panel of individual mice loaded lane by lane on the gel (see Figure 1).
Fig. 5 shows a panel of individual 6 frames taken at 30 s intervals throughout these experiments during which drug (or vehicle) treatments were administered.
PCR with each pair of primers was performed in 96-well plates using genomic DNA from a large panel of individual mutant embryos and WT embryos as controls.
Because of the large volume of sample required and ethical restrictions, the use of a panel of individual patient sera was not possible or practical.
