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Starting from 100 ng of DNA, MiSeq 54-gene panel library preparation and sequencing were performed according to the manufacturer's protocol as described previously (Luthra et al, 2014).
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The Ion AmpliSeq panel libraries were sequenced on an Ion PGM, which was carried out by Life Technologies team in Paisley.
MiSeq cluster densities of 811 K/mm and 880 K/mm were achieved for the Fluidigm custom panel libraries prepared using fresh gDNA and FFPE gDNA, respectively.
The Fluidigm custom panel and the SureSeq panel libraries were sequenced on the Illumina MiSeq (Fresh and FFPE gDNA were sequenced separately for each of the panels), which was carried out in the Translational Genomics Unit at St. James's University Hospital.
The apartment's formal dining room and ornately paneled library overlook a south-facing terrace.
Weston $1.565 million 70 Eleven O'Clock Road 4-year-old wood colonial; family room, 4 fireplaces, paneled library, 3-car garage, 2.15-acre lot; taxes $40,097; listed at $1.499 million (multiple bids).
The home's entertaining spaces are on the lower of the two floors and feature a formal dining room, paneled library and a living room with a 13-by-10-foot 13-by-10-foot 13-by-10-foot 13-by-10-foot
Scarsdale $1.225 million 5 Walworth Avenue, Westchester r 5 f 4 h 1 35 weeks 94-year-old stone and stucco Tudor; fireplace, paneled library, wraparound deck, basement, 2-car garage, 75- by-100-ft.
Greenwich $3.8 million 10 Indian Chase Drive 5 4 1 2 weeks 90-year-old wood colonial renovated in 2000; family room, paneled library, 5 fireplaces, c/a, access to private beach, 1-acre lot; taxes $21,486; listed at $3.9 million.
doormen, concierge, dining room, renovated kitchen with granite countertops, paneled library, crown moldings, common deck, health club, pool, sauna and party room in building; common charge $814; taxes $13,008; listed at $1.8 million, 2 weeks on market (Brokers: Halstead Property Co.; Brown Harris Stevens) UPPER WEST SIDE $420,000 345 Riverside Drive (107th St).
More than $1.2 million Wilton $2.3 million122 Powder Horn Hill Road Number of bedrooms: 6 Number of full bathrooms: 5 Number of half bathrooms: 2 Weeks on market: 39 weeks New wood and stone colonial; family room, paneled library, 3 fireplaces, finished basement, c/a, 3-car garage, 2.03-acre lot; taxes not yet assessed; listed at $2.345 million.
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