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As shown in Figure 2b (left panel), ChIP assay showed that the HIPK2 recruitment onto MT2A promoter was hampered in HEK-APP cells in comparison with HEK-293 control cells, whereas it was recovered by treatment with β secretase inhibitor.
(B ) Upper panel, ChIP seq demonstrates SOX2 binding to BIM and BMF.
For the correlation between multiple genetic biomarker panel (chip) results and CCRT response, negative chip results were more significantly correlated than positive chip results to poor CCRT responses (TRG 0-1; P < 0.001 in univariate analysis and P = 0.014 in multivariate analysis; Tables 3 and 4).
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The input-normalized ChIP-seq signals around the TSS reveal markedly increased binding of MOF for male X-linked fly genes (left panels) that was not recapitulated in the mouse (right panels; ChIP-seq signals from ESCs).
The best part was that we could put the whole thing on magnetic tape, which could be read by an automatic wiring machine that actually made the connections on the back of a huge panel of chip sockets.
An even smaller subset of SNPs (N = 16) are included on the widely used Illumina HumanHap610 fixed panel GWAS chip.
While not included in the decision-making process in this clinical trial, the Ion Torrent Cancer Panel gene chip was performed to assess use in future studies.
Indeed, immunoprecipitation with the SOX2 antibody followed by the c-MYC antibody led to the detection of a number of promoters in undifferentiated BG01V cells (Fig. 6B, top panel, "Sequential ChIP: SOX2 > c-MYC").
The same promoters were detected if the NANOG antibody preceded the c-MYC antibody (Fig. 6B, middle panel, "Sequential ChIP: NANOG > c-MYC") or if the OCT3/4 antibody preceded the c-MYC antibody (data not shown).
Second, only SOX2 and OCT3/4 co-occupy the SUZ12 promoter [ 10], and consistent with that, only immunoprecipitation with the SOX2 antibody followed by the OCT3/4 antibody lead to the detection of the SUZ12 promoter (Fig. 6A, middle panel, "Sequential ChIP: SOX2 > OCT3/4"), whereas other antibody combinations did not.
The National Semiconductor Corporation cut its first-quarter sales forecast yesterday, citing customer efforts to cut inventories and weaker-than-expected orders for flat panel display chips and cellphone parts in China.
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