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For the type II system there is evidence that Cas9 is integral in the initial recognition of the potential protospacer + PAM sequence in the invading DNA [ 15].
Green, PAM sequence.
PAM sequence are labeled in green.
AGG (red) is the protospacer-adjacent motif (PAM) sequence.
The sequence of gRNA is shown with the PAM sequence.
Double peaks near the PAM sequence (green) are indicated.
The sgRNA PAM sequence is labeled in red.
This section computes the second- and fourth-order cumulants of a binary PAM sequence.
The mutated PAM sequence and its reverse complement sequence are highlighted in yellow.
The sgRNA sequencing and the PAM sequence are shown in black and blue, respectively.
Control, amplified products from target regions with no double peaks near the PAM sequence.
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