10.7554/eLife.06813.008 Figure 4. Genetic crosses between the pale beach mouse P. polionotus leucocephalus (top row left) and the darker mainland mouse P. p. polionotus (top row right) result in first-generation F1 hybrids, all with intermediate coloration (second row).
A null mutation of Fig4 in the pale tremor mouse results in the formation of vacuoles in the cell bodies of neurons of the central nervous system (CNS) and peripheral nervous system, with extensive degeneration of sensory neurons of the dorsal root ganglia (DRG) and juvenile lethality around the time of weaning (Chow et al, 2007).
The mouse pale ear mutant, HPS1 ep, is the model for the most common HPS disease, HPS 1, while the pearl mutant, Ap3b1 pe, is the model for HPS 2. As for all HPS genes, the products HPS1 and HPS2 (a subunit of the AP-3 adaptor complex, Feng et al. 1999), respectively, are involved in vesicle trafficking and the secretory process, and known to influence separate pathways (Feng et al. 2002).
Although the liver looked pale in these mice, no abnormalities were observed and no cleaved caspase 3 positive cells were seen in this organ, suggesting that THOC5/FMIP depleted mice may have anemia, and/or internal bleeding.
Then, 5-10ml of the DiI solution was injected at a rate of 1-2ml/min for 5-10 min. Successful perfusion was monitored by observing color change in the ear, nose and palms of the mouse from pale while to purple.
5.At this age, I especially love dawnOn the sea, stars above the trees,Pages in "The Threefold Life,"And the pale faces of baby mice.
Lungs of Creb1−/− fetal mice were pale in colour and smaller than wildtype controls in proportion to their reduced body size.
Secondly, although we find that the lung from Creb1−/− mice are pale in colour, the respiratory defect we observe is unlikely to be due to impaired pulmonary circulation as we show that loss of Creb1 did not seem to affect development of the lung vasculature.
These data also agree with the observation that liver and spleen in THOC5/FMIP deleted mice appear pale.
Strikingly, the plasma collected from Arfrp1 vil−/− mice 2 h after the oil bolus appeared to be transparent and bright, while plasma from Arfrp1 flox/flox mice was pale and turbid (data not shown).
