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For a sample size of 8 pairs, samples that have 7 increases and 1 decrease or 1 decrease and 7 increases occur (that is, has a p-value of) 5.46% of the time (see Additional file 1: Figure S1 on the paired Wilcoxon distribution).
Figure 2 showed the IBS of 2 matched pairs (samples 76629543 and 2000619) in NGS across the genome.
Urine-saliva pairs samples of pregnant women in the second and third trimester, and saliva only in the first trimester were collected.
Due to the small sample size of this sub-dataset, with only 8 (4 pairs) samples, only 7 genes were identified as DEGs with a non-zero score.
We evaluate the prediction accuracy of the supervised method by generating labeled feature vectors for all gene pairs (samples) of a network.
Excluding 8 (4 pairs) stage I samples, we selected 38 (19 pairs) samples from this dataset: 16 (8 pairs) stage II, 14 (7 pairs) stage III, and 8 (4 pairs) stage IV samples.
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This explains the lower correlation scores observed both in non-paired samples and in paired samples.
* Wilcoxon's test for paired samples.
Significance of differences in means of paired samples was assessed using paired-sample t-test.
Student t tests for independent and paired samples were used.
Wilcoxon signed-rank test was used for nonparametric paired samples.
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CEO of Professional Science Editing for Scientists @ prosciediting.com