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The probes designed for each of the 500 pairs of targets overlapped 465,034 bp, or 85.6% of the targeted sequences, and were synthesized on HD2 (HX12) arrays, each slide consisted of 12 subidentical subarrays each with 135,000 probes designed against the 550-Kbp transcript space.
Unlike most previous approaches which only focus on producing appearance models for all targets, we further consider discriminative features for distinguishing difficult pairs of targets.
In our test case, there are three pairs of targets at different ranges from radar, where each pair consists of two targets that are equally distant from each other at every of the three different ranges (Figure 5).
We have also investigated the correlation between scores for other pairs of targets; the independence of scores between SMPD and LYPLA1 has an associated p-value of 0.507, and hence at the 5% level the null hypothesis that they are independent is not rejected.
Forty pairs of targets (T1-T2) were created using 4 to 7 letters words.
Surprisingly, we detected statistically significant differences in mean transcript levels for only 10 pairs of targets (corresponding to A. thaliana genes At1g11840, At1g64720, At1g72290, At1g76180, At2g02990, At2g24420, At4g33150, At4g39090, At5g24770, and At59310; see: Table S5).
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In this detection method, the difference in only a few base pairs of target DNA can be discriminated and specific detection of Microcystis spp. was achieved.
A total of 32 hybridizations were performed, which included the four pairs of target organs (male and female) from four biological replicates, all hybridized against the common reference.
We designed three pairs of targeting hooks to investigate the selectivity of the new TAR cloning system.
The pairs of target residues were replaced with cysteines, and the side chain positions were subsequently optimized by using SCWRL 4.1.
Differences in DNA adduct levels among the organs (1-way ANOVA) or between pairs of target and non-target organs (Tukey-Kramer test) were not significant (p > 0.05).
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