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VH-Vλ pairings were observed in frequencies between 0.1% and 1.8% in the unselected HAL9 with VH3-30/Vλ6-57 being the most abundant combination.
Three successful pairings were observed among six isolates from Leigh Creek-Arkaroola, while the remaining isolates were incompatible with each other.
Evidence supporting this hypothesis is obtained using comparative syntenies within salmonids is as follows: Within the salmonids the following zebrafish and medaka homology pairings were observed within linkage group arms: Dr8 with Olat9, 2×; Dr5/10/21 with Olat12, 3×, Dr5/10/15 with Olat13, 1×; Dr5/10/21 with Olat14, 2×; Dr14 with Olat10, 2×.
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The occurrence of (seemingly) energetically feasible non-native pairings was observed across all families studied here, but we found that non-native pairings with low energies are quite common between nuclear receptors (NR) and cofactor peptides.
As in the cross-phylogeny bioassay, the presence of discoloration (34.7% of pairings) was observed only in pairings with small zones of inhibition (i.e., when there was substantial growth of the cultivar) and appeared to be present primarily in pairings involving three of the Pseudonocardia strains (Table 1: B, I, or L).
The abundance of heavy- and light-chain pairings that were observed in the unselected libraries and within selected antibodies are illustrated in Figure 4 for VH-Vλ combinations and in Figure 5 for VH-Vκ combinations.
The T3 T5 (m/z 1567.78), T5 T11 (m/z 1578.68), T3 T12 (m/z 2005.00) and T11 T12 (m/z 2015.90) pairings indicated scrambling, but were observed only in the PMF spectra acquired from the sample trypsinized in the basic buffer condition (20 mM ammonium bicarbonate, pH 8.0, for 2 or 6 h).
Modest correlations (~ 30%) were observed between the pairings of PFHS PFOA, PFHS PFNA, PFOA PFOS, and PFOS PFDA.
Clamp connections were observed in two within-species pairings of A. hinnulea (Table 5).
Of the gene pairings that are observed among the carbohydrate metabolism, only the GAL1, GAL7, GAL10 genes are found as immediate adjacent neighbors in species other than S. cerevisiae.
Intriguingly, homeologous pairings have been observed to include at least one metacentric chromosome (Wright et al. 1983), and duplicated markers map to such chromosomes (Brieuc et al. 2014), supporting the view that metacentric chromosomes play an important role in homeologous pairing (Phillips et al. 2009; Brieuc et al. 2014).
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