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On paired trials the interstimulus interval (ISI) between the CS and US onset was 350 msec.
When a recording was stable, the animal was subjected to blocks of paired trials.
All salient aspects of conditioning were shown by analysis of paired trials.
In one case, we were able to record a block of paired trials during the recording of an MLI immediately adjacent to a Purkinje cell from which we also recorded a block of paired trials.
The data from 85 paired trials were collapsed to remove the last 5 trials, together with 10 CS alone trials and 5 US alone trials thus leaving 8 blocks of 10 paired trials to analyse.
We originally analysed the data in blocks of 5 paired trials however there was no meaningful difference between the analysis in blocks of 5 or 10.
Similar(40)
To control for unforeseen bias in the female preference observation enclosure, males from a given lineage were alternated between sides 1 and 2 after each paired trial.
However, in that study a deviant paradigm was used, in which a train of stimuli was used instead of the classical paired trial.
Trials were rejected if they had eyeblinks in the baseline period, responses immediately after the onset of the CS (see below for Alpha response), large eye opening in CS period or the absence of a UR on a paired trial.
Previous research showed that this design leads to a significant increase of brain activity, startle reflex potentiation, SCRs, and self-reported negative affect and arousal in healthy controls during pairing trials compared to habituation trials (Anders et al., 2005).
Delay conditioning consisted of 100 trials of which eighty trials were paired, ten trials were CS alone and five trials were US alone.
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