Asynapsis can reflect pairing difficulties of chromosomes involved in certain rearrangements, such as translocations, inversions, or other structural mutations, or can be caused by mutations in certain meiotic genes.
Another issue is the fact that manually obtaining a suitable ground truth dataset is, as has previously been discussed, a far from trivial task paired with many difficulties.
Three methods are following this concept by either looking at recurrent splits within single reads, misalignment of paired reads or overall difficulty in mapping at a given location.
However, low contrast was observed when C-S-H and monosulfate were paired together leading to difficulties segmenting in an unbiased manner.
Prevented from pursuing her doctorate because of financial difficulties, she paired her interest in marine biology with her writing talent to get a job with the U.S. Fish and Wildlife Service, producing brochures and pamphlets, and also pursued an independent writing career.
If you are having difficulty pairing your devices, there are a variety of articles on wikiHow that can help you get it all sorted out.
I hated the silent protagonist of Origins, and the dice-rolly combat system that felt dated even back then, and was frustratingly paired with a wildly uneven difficulty curve.
The main difficulty was obtaining paired blood and tumour DNA samples, mainly because of the low incidence of such sarcomas.
However, deciphering growth factor receptor kinase pathways at the time of acquisition of tamoxifen resistance has proved notoriously problematic in clinical material because of the difficulty in obtaining paired samples before tamoxifen administration and subsequently on relapse.
Despite accelerated research in this field, the mechanisms associated with lower-grade gliomas have not been clarified, partly because of difficulties in obtaining paired initial tumor tissue and recurrent tissue samples.
Our study underscores the difficulties in assessing paired primary and recurrent tumour specimens when analysis is performed in a retrospective fashion with 26% of specimens having insufficient material available for in situ hybridisation.
