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Integration is driven by the bacterial Xer recombinases, which catalyse the exchange of a single pair of strands between the phage single-stranded DNA and the host double-stranded DNA genomes; replication is thought to convert the resulting pseudo-Holliday junction (HJ) intermediate into the final recombination product.
They figured out that DNA was a pair of strands twisted around each other.
A short pair of strands in HadA was undefined in the native structure because of poor density.
If different pair of strands are cleaved at each junction, there is a reciprocal exchange of DNA arm flanking the site of recombination generating crossover recombinants (CO).
Our modification allows using the algorithm for pair of strands.
TmPurQ contains a classic triad glutaminase fold; it consists of a nine stranded mixed β-sheet flanked by four helices, a pair of strands, and four 310 helices.
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Weight figures are usually estimates, but in 1937 a pair of stranded male sperm whales were measured intact in the Netherlands: one was 16 metres (52.5 feet) long and weighed 39 tons, and the other was 18 metres (59 feet) and 53 tons.
He was placed in command of a detachment of the High Seas Fleet, composed of two battlecruisers, eleven battleships, four light cruisers, and twelve torpedo boats, sent to Denmark to retrieve a pair of stranded U-boats in November 1916.
XerD generates Holliday junctions by creating a pair of strand exchanges and XerC resolves this structure through the reaction between directly repeated dif sites in circular DNA [ 60].
Resolution of the resulting pseudo-HJ by a second pair of strand exchanges would lead to the formation of a lethal linear covalently closed dsDNA chromosome (Bouvier et al, 2005).
Similarly to CTXφ, VGJφ-integration results from a single pair of strand exchanges, which is catalysed by XerC, and the resulting pseudo-HJ needs to be converted into product by replication (Das et al, 2011b).
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