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A coisogenic pair of strains differs only at a single genetic locus.
Comparison of a coisogenic pair of strains is a powerful method for detecting the effects of a single gene.
To test this prediction and the generality of selection inversion with respect to mechanism of resistance, we applied our assay to another pair of strains, in which tetracycline resistance is mediated by a different mechanism.
The Mantel test of matrix correlation indicated asymmetry in pairs of strains; the same level of inhibition was not necessarily obtained when switching the order of inoculation of a given pair of strains (Mantel r = −0.09; p = 0.8562).
The six strains from each group were randomly hybridized with six strains from every other group; each hybridization compared a pair of strains that differed in either clade or stx profile, or both.
Finally, in order to test if the same level of inhibition was obtained when switching the order of inoculation of a given pair of strains (i.e. if reactions displayed were symmetrical), Mantel tests of matrix associations with 10,000 permutations were performed on reactions displayed in both bioassays using Arlequin 2.0 [63].
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In the magnificent "Diana of the Uplands," the Sargent protégé Charles Wellington Furse paints his wife holding back a pair of straining greyhounds on a windy day in the country, her dress and hat-ribbons flying.
A heterozygous pair of strain DBA/1-Abca1tm1Jdm (stock number 003897) mice was purchased from the Jackson Laboratory (Bar Harbor, ME) and maintained on regular chow and water ad libitum, in accordance with the institutional guidelines of the Animal Care and Use Committee.
The algorithm then compares pairs of strains, looking for SNP patterns that are shared among strains with similar phenotypes, but not shared among strains with different phenotypes; the more strains that can be compared, the better the prediction.
Microarrays were performed between overlapping pairs of strains in a dye-swapped design.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com