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This region includes a pair of essential palindromic sequences that are evolutionarily well-conserved and the first of these is likely to form an intra-repeat hairpin structure.
The conclusion on the monophyly of the bikonts rests, primarily, on the fusion of a single pair of essential genes, those for dihydrofolate reductase (DHFR) and thymidylate synthase, purportedly, buttressed by the analysis of domain architectures and sequence-based phylogenies of some highly conserved proteins, such as myosins (Richards and Cavalier-Smith 2005).
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Myosin II is a hexamer composed of two heavy chains of ∼200 kDa and two pairs of essential and regulatory light chains.
In agreement with previous results, intraoperonic pairs of essential and of highly expressed genes are more conserved.
Both pairs of essential and nonessential genes tend to be less conserved in the operons of larger genomes (fig. 3 A– C).
We classed genes as essential or nonessential using E. coli data (Baba et al. 2006), and tested whether pairs of essential genes (EE) are more often conserved in the same operon than pairs of nonessential genes (NN).
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Note that SLs are pair of non-essential genes whose simultaneous removal can lead to zero growth (Suthers et al. 2009).
Synthetic lethal (SL) screening was also performed to identify the pair of non-essential genes whose simultaneous deletion become lethal, revealing the existence of more than 220 pairs of SLs on rice central metabolism.
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A salad bowl and a pair of servers are essential tabletop items, but are rarely designed as a unit, said Paul Rowan, a founder and vice president for design of Umbra, the home furnishings company.
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