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The criteria to choose the best pair of alignments for the ends are then dictated by the user, and can be based on best-combined score, uniqueness, as well as other post-alignment filtering criteria.
Case III: A*(C) is a pair of alignments; the contig is considered a potential split-candidate (see below).
Each candidate is made up of a contig and a pair of alignments associated with that contig.
Additionally, it uses an elaborate probabilistic model that incorporates pairing information to compute for a pair of alignments of a read pair, the posterior probability of having predicted an incorrect alignment.
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For each pair of reads, the aligner produces a pair of alignment records.
The effective length factor is obtained by solving the exact equations numerically which require many routine calculations or by using a pair of alignment charts for the two cases of braced frames and sway frames.
An all-vs-all comparison was performed among each pair of alignment assemblies within each cluster.
Normalized mutual information (NMI) was calculated by dividing the MI score for each pair of alignment positions by the joint entropy of that pair of positions.
In addition, GU pairs have an asymmetric effect in multiple sequence alignments: Suppose a particular pair of alignment columns exhibits a GC → GU substitution in one reading direction; this preserves base pairing and hence is consistent with a conserved structure.
The latter is challenging because, in the SAM format, an alignment of a read pair is described by a pair of alignment records, which cannot be expected to be adjacent to each other.
Using the above strategy, we inspect for every single alignment column the probability of observing SNDs and LPDs and for each pair of alignment columns all four combinations of probabilities of observing phased SNDs and LPDs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com