Sentence examples for pair confirmation from inspiring English sources

The roots of Arg7 were sampled on 120 DAP, 180 DAP, 210 DAP and 240 DAP for expression profile analysis of miRNA and targets by real-time PCR, and miRNA-target pair confirmation by RLM-RACE.

On the basis of the miRNA:target pair confirmation and the co-expression analysis, 13 miRNA:target pairs were chosen for further assays of their regulatory relationship by quantitative real time PCR (qRT-PCR) in Arg7 storage roots at different growth stages; the corresponding primers are listed in Additional files 2 and 6.

But the relative lack of specificity obtained through using this primer pair requires confirmation, such as the sequencing of the obtained amplicon.

Because the distribution for each gene is different, gene pair duple confirmation refers to a gene pair (A, B) could be identified only when gene A is chosen, the pair shows significant co-expression change; meanwhile, when gene B is chosen, the pair is also determined with significant differential co-expression between two progressive stages.

This lack of specificity could be bypassed on the basis of the age of the animal at the time of sampling, as PoNoV infections have usually been found in older animals [ 31] or by usage in serie of a pan-calicivirus primer pair for confirmation, as the SR80/JV33 primer pair [ 32].

The primer pairs for confirmation of targeting of the loxP cassette into the HAC were: HRL-F/TKpAF (4.1 kb), 5′-CAGAGCCAGACAGGAAGGAATAATGTCAAG-3′ and 5′-CCGGACGAACTAAACCTGAC-3′; PGKr-3/HRR-R (3.5 kb), 5′-CCAGAGGCCACTTGTGTA-3′ and 5′-GCATCTCAATTAGTCAGCAACCATAGTCCC-3′.

This is the reasoning for identifying gene pair with dual confirmation.

Specificity of each primer pair was confirmed by melting curve analysis and agarose gel electrophoresis, and PCR products were sequenced for product confirmation.

This analysis of the individual (A,A) covariation pairs provides independent confirmation that these specific residues are positively selected for drug-resistance.

To confirm altered expression of 4 genes, an additional 6 matched pairs (n = 13) were included in relative real-time PCR confirmation studies.

A total of 60 primer pairs were prepared for confirmation of 28 putative terminators (Additional file 1).