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2-D PAGE was prepared and run as previously described [ 48].
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About 90 percent of the obituaries that make the front page are prepared in advance.
A native polyacrylamide gel electrophoresis (native-PAGE) was prepared as previously described [ 15].
SDS-PAGE was prepared with 15 18% acrylamide, and tricine SDS-PAGE was performed as described by Sousa etricine SDS-PAGE
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Samples for two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) were prepared as previously described [ 56, 69].
Samples for BN-PAGE were prepared as previously described with minor modifications.
Protein samples for SDS-PAGE were prepared from the cell extract after sonication and centrifugation.
Samples for SDS-PAGE were prepared in Laemmli buffer normalized to equal protein concentration.
Samples for SDS-PAGE were prepared at 1 μg protein/μl concentration using 5× SDS-PAGE buffer (Pierce).
PAGE gel was prepared from 19 1 acrylamide/bisacrylamide.
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