When SaOs-2 transduced cells were exposed to 15 μM FTI-277, a mobility shift on SDS-PAGE was observed in HDJ-2, reflecting inhibition of FTase.
Although an anomalous migration in SDS-PAGE was observed in the VHH 3B2, this characteristic does not seem to be caused by this modification more than by another post-transductional modification since no glycosylation was detected when using the GLYCOPRO Detection Kit (Sigma, USA) (data not shown).
Reproducible protein patterns via one-dimensional SDS-PAGE were observed across all samples (data not shown).
For each parameter, its influence on the CPU usage and on the number of pages being read (page-in) and written (page-out) was observed.
The most significant result obtained by SDS-PAGE analysis was observed in the group of operated rats treated with saline, where the expression of 6 protein bands was significantly increased, compared to rats treated with Indomethacin.
(what else could be observed?); page 10 "some difference was observed among these genes"; page 11 "most of the genes had a different response to the two treatments (Fig. 11a, b).
3) page 10 "some difference was observed among these genes"; page 11 "most of the genes had a different response to the two treatments (Fig. 11a, b ).
However, some genes showed similar responses to the two treatments…" (following text to the end of the paragraph non-informative); page 10 "some difference was observed among these genes"; page 11 "most of the genes had a different response to the two treatments (Fig. 11a, b).
From SDS-PAGE analysis, it was observed that few bands (around 45 60 kDa) diminished in the UBP fraction as compared to fraction 1 (Additional file 1: Figure S8).
Although a consistent 2D-PAGE distribution pattern was observed in all the samples from the two cell lines, different results might be expected when analyzing patient material.
The purified esterase B1 was analyzed by SDS-PAGE anative-PAGEand, and one major band was observed after PAGE under denaturing and nondenaturing conditions.
