The increased proteolytic activity correlates well with the increase in the protease content (see SDS-PAGE analysis Figure 2a, b; the whole SDS-PAGE picture is available in the Additional file 1: Figure S1). Figure 2 Activity studies and SDS-PAGE visualization of produced CelA2, BSLA, subtilisin Carlsberg wild type.
Immunoprecipitates were washed three times with 50 mM Tris-HCl pH 7.4, 137 mM NaCl, followed by denaturing SDS-PAGE, visualization by autoradiography, and quantitation using a Molecular Dynamics PhosphorImager and ImageQuant software.
Product separation by SDS-PAGE, visualization by fluorescent scanning, quantification, and normalization within each lane were performed as described for the substrate selection experiments.
The high-spatial reproducibility and quantitative reproducibility resulted from the same experimental condition maintained for each sample analysis, including sample preparation, IEF, SDS-PAGE, visualization, gel digitalization, and image analysis.
Therefore, we performed single-nucleotide-incorporation experiments followed by analysis through denaturating polyacrylamide gel electrophoresis (PAGE) and visualization using autoradiography.
When the user eventually moves on to the results page, the visualization for the data is an implementation of Data Drive Documents (D3), a JQuery library [ 26].
Immunoprecipitates were washed three times with 50 mM Tris-HCl pH 7.4, 137 mM NaCl, followed by SDS-PAGE and visualization by autoradiography.
Cells were homogenized in lysis buffer (50 mM Tris 7.5, 150 mM NaCl, 1% Triton X-100, 100 µM NaF, 0.5 mM Na3VO4, 10 mM β-glycerol phosphate), followed by anti-Flag immunoprecipitations, SDS-PAGE, and visualization using a Molecular Dynamics PhosphorImager.
For microarray data, the user can browse and search expression information on the microarray visualization page and microarray search page.
The visualization page includes three views of the data: a clustered heatmap, a similarity matrix heatmap of the columns, and a similarity matrix heatmap of the rows (not shown).
The Output Visualization page presents the climatology results, generated from the post-processing of the simulation outputs.
