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It was quickly noticed that the first page of some journal folders was discoloured possibly due to the interaction with the cardboard of the folder and therefore "front page" sampling was made within the first five pages at the front of the folder.
Reactions were stopped by adding equal volume of 2X SDS PAGE sampling buffer.
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Page after page samples many of the Italian master's 298 typefaces.
Attending review of random page samples correlated well with resident review.
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Reactions were stopped by the addition of polyacrylamide gel electrophoresis (PAGE) sample loading buffer.
The nuclear, mitochondrial and plasma membrane pellets were resuspended in SDS PAGE sample buffer.
After SDS 12% PAGE, samples were blotted on polyvinylidene difluoride membranes (Bio-Rad) and blocked with 5% bovine serum albumin.
The magnetic beads were rinsed with 2× 100 μl of 0.1% CHAPS in buffer and the bound protein was extracted by incubation in 20 µl SDS-PAGE Sampling Buffer (BioRad) for 5 min at room temperature and an equal amount of Sampling Buffer with 2-mercaptoethanol was then added.
Those who weren't thumbing pages were sampling oysters, sushi rolls and sliders.
The stimulated cells were rinsed with ice-cold PBS and lysed in SDS-PAGE sample buffer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com