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After SDS-PAGE of samples, the fibrin-zymography was tested on agarose fibrin plates.
SDS-PAGE of samples was carried out (NuPAGE Novex BIS-TRIS 12 14% gel; Invitrogen Ltd).
SDS-PAGE of samples was carried out (NUPAGE Novex Bis-Tris 4 12% gel; Life technologies, Paisley, UK).
Secretion of recombinant proteins into the medium was confirmed by SDS-PAGE of samples from transfected and non-transfected cells.
SDS-PAGE of samples collected during the isolation procedure showed that rSbpA-GG had accumulated in the insoluble fraction of the lysed E. coli BL21 (DE3) Star host cells (data not shown).
The document included two pages of sample questions council executives might be asked by local reporters after the Times article ran, and suggested answers.
The experiment consisted of task pages presented sequentially to the test subject, each page containing one pair of samples or an individual sample depending on the test setup.
The results of spectral analysis indicating HmuY-haem complex formation were corroborated by non-reducing SDS-PAGE analysis of samples obtained at different time intervals during incubation of HmuY with immobilized methaemoglobin.
Figure 6B shows SDS-PAGE profile of samples before and after cIFN-α purification.
SDS-PAGE analysis of samples taken from this step showed that the purity of SUMO-FGF21 reached 96.5%.
An SDS-PAGE analysis of samples from the different purification steps for HIS-SUMO-CP4 indicated that a significant level of purification was achieved (see Additional file 2).
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