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Participants evaluated the latter symptom using a balance and pads of three sizes supplied by the Study Centre and reported the weight of soiled pads by size in the electronic diary.
D. Percentage of tumors formed by varying numbers of Py230 cells injected in 2 mg/ml matrigel into the cleared mammary fat pads of three to four-week-old female mice.
For transplantation assays, freshly harvested cells from the Lin-CD29hiCD24+ Lin-CD29hiCD24+ransplanted in a volume ofractioninto the de-epithelialized fourth mammary fat pads of three were-old syngeneic mice.
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Adipose tissue was harvested from the inguinal fat pads of five male BALB/c mice (Jackson Laboratory) at the age of 8 12 weeks and pooled for further processing.
When non-targeted MCF7 cells were injected into the mammary fat pads of eight oophorohysterectomised female mice all produced primary tumours.
One, four, or ten million cells expressing GFP-luciferase were injected directly into the surgically exposed #4 mammary fat pads of six to eight week old female SCID-Beige mice.
Progression of arthritis was assessed with a non-automated 'cat-walk' walking gait test, which measured the distance between the central pads of two alternating hind footprints (Parvathy and Masocha, 2013).
DNase I hypersensitivity peaks data generated by ENCODE project from gonadal fat pads of two male B mice was downloaded through tables tool at UCSC genome browser (http://genome.ucsc.edu/ENCODE/, wgEncodeUwDnaseGfatC57bl6MAdult8wksPkRep1.narrowPeak.gz and wgEncodeUwDnaseGfatC57bl6MAdult8wksPkRep2.narrowPeak.gz).gz
For tumorigenesis assays, M3 or M4 cells were suspended in serum-free DMEM/F12 medium, and 5 × 10 cells were injected into the #2 and #7 mammary fat pads of six- to eight-week-old female athymic NCr nu/nu mice.
For MCF7 tumors, 2 × 10 MCF7-EGFP cells were mixed with BD Matrigel™ Basement Membrane Matrix BD Biosciencess, cat. no. 356234) and injected into the left and right cleared fourth inguinal mammary fat pads of two female athymic Nude-Foxn1nu mice (Harlan Laboratories).
To generate ER- and PR-positive tumors, 10 or fewer Py230 cells were injected in 2 mg/ml matrigel into the thoracic or inguinal mammary fat pads of six to eight week-old feight week-old
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