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The effect of oxygen deficiency was confirmed by increasing the air circulation rate in front of the catalyst pad using a fan.
In this study, we investigated the therapeutic efficacy of PVAX nanoparticles for PAD using a cell culture model and a mouse model of hindlimb ischemia.
MDA-MB-231 parental or clonally-derived cells (106 cells in 100 µL PBS; n = 10 mice per cell line) were inoculated into the upper mammary fat pad using a 27-gauge needle.
Following appropriate institutional animal care committee approval, four-week-old Balb/c mice were injected transdermally with the G3- and vector-transfected 66c14 cells (1×107 cells in 150 µl 10% FBS/DMEM medium) into the fourth (inguinal) mammary fat pad using a 1 ml syringe with a 26 G needle.
On day 0, β-Gal control or PERKΔC-expressing MCF10A cells were suspended in serum free media in a 50-µl volume (3×106 cells/mouse) and injected in the cleared fat pad using a 25 gauge needle to avoid damaging the cells.
The solution was recovered by pressing the pad using a pipette tip.
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Mouse gonadal WAT was homogenized in lysis buffer (~ 1 ml/fat pad) using an Ultra Turrax Homogenizer (Fisher Scientific, Waltham, MA).
Genomic DNA was isolated from ground cartilage, synovium or infrapatellar fat pad using an EZNA DNA/RNA isolation kit (Omega Bio-Tek), as previously described (24).
It's as close to drawing on paper as any digital pen or tablet I've ever tried, and my first Wacom pad used a serial port, so I've been around a bit.
Never heat water source with a reptile heat pad, use a aquarium heater instead.
For cats who like to scatter litter by jumping out of the litter box like it's a launch pad, use a covered litter box and place it so that the entrance faces the wall.
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