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Right panels: Diagrams showing take rate and fat pad filling.
Faster mammary fat pad filling and excessive ductal side-branching result from increased mammary stem and progenitor cell populations/activity, respectively.
In contrast to our observations in developing MGs from Becn1 +/− mice (Fig. 2A and B ), whole MG mounts revealed no difference in ductal side-branching between Atg7 +/+ and atg7 F/F; Krt14- Cre mice and, more impressively, showed delayed rather than accelerated fat pad filling in 6.5-wk-old atg7 F/F; Krt14- Cre mice (Fig. S4C and S4D).
Mammary fat pad filling was quantitated comparing total fat pad area and outgrowth area using Image J software.
For mammary fat pad filling and ductal side-branching cohoused littermates (for pubertal time points) and cohoused (for at least 3 mo after last pregnancy) multiparous mice were compared.
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cPositive outgrowth defined as ≥ 25% of fat pad filled; dpositive outgrowth defined as ≥ 10% of fat pad filled; epositive outgrowth defined as ≥ 3% of fat pad filled.
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