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These observations are consistent with burial of these probes in a collapsed, but loosely packed intermediate, followed by the rate-limiting formation of the densely packed native core, which brings the tryptophan residues into close contact with intramolecular quenchers.
Examples of such protein crystalline arrays that have been visualized with subnanometer resolution include Bacillus S-layers [13], the hexagonally packed intermediate (HPI) layer of Deinococcus radiodurans [14], purple membrane from the archeon Halobacterium [15] and porins crystals of Escherichia coli [16].
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We herein demonstrate that, during targeting, most mature domains maintain loosely packed folding intermediates.
The present work is experimental evidence for the proposed final "locking" mechanism in GFP folding forming an incorrectly or loosely packed barrel under intermediate (hysteretic) folding conditions.
Hundreds of villagers packed into the Guangzhou intermediate people's courthouse to witness the proceedings.
We illustrate these concepts with an extensive case study on dimethyl ether production using an intensified process featuring a dividing-wall distillation column and an adiabatic packed bed reactor with intermediate quenching.
Next follow tower internals damage, abnormal operation incidents (startup, shutdown, commissioning), assembly mishaps, packing liquid distributors, intermediate draws, misleading measurements, reboilers, and explosions.
A set of 80 numerical simulations was conducted to represent conditions in a 2-D intermediate-scale, heterogeneously packed test aquifer with the dimensions of 9.5 m long 1.0 m high and 5.0 cm thick.
When receiving the PACK message, intermediate CRs look up the routing table again.
If the tentative entry exists that corresponds with the name prefix in the received PACK message, intermediate CRs update the status of the path entry as "valid".
Furthermore, the critical path is analyzed and appropriate optimization techniques (such as DSP register packing and intermediate register insertion) are applied to the best candidates of the architectures mentioned.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com