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Then 25 µl of 5% sodium citrate was added and incubation was continued at 56°C for 30 min. Each serum sample was allowed to cool to room temperature and 100 µl of packed chicken RBCs were added.
Heat-inactivated sera were treated with receptor-destroying neuraminidase (RDE) (Roche Diagnostics, Indianapolis, IN) overnight at 37°C, heat inactivated for 30 min at 56°C and incubated with packed chicken RBC for 1 h at 4°C to remove any cryoglobulins interfering with the agglutination reaction.
Then the required final dilution of the treated antiserum (1 10) was prepared and adsorbed with packed chicken erythrocytes.
45 In brief, serum samples were treated with receptor-destroying enzyme and heated for 30 minutes at 56°C to remove non-specific inhibitors, adsorbed with packed chicken erythrocytes to remove non-specific agglutinins, and assessed in the HI by using 4 HA units.
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With his friends Walter (Judge Reinhold) and Lewis Charles Estenn), he packs chicken eggs in a swirl of feathers.
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