Sentence examples for packaging sequences from inspiring English sources

These HIV-1 packaging sequences constitute 19.6% of the wild-type HIV-1 genome and contain functional cis elements that potentially compromise clinical safety.

This has been achieved by reconfiguring the vector to mediate reverse-transcription with a single strand transfer, instead of the usual two, and in which HIV-1 packaging sequences are not copied.

Here, we describe the development of a novel lentiviral vector (LTR1) with a unique genomic structure designed to prevent transfer of HIV-1 packaging sequences to patient cells, thus reducing the total HIV-1 content to just 4.8% of the wild-type genome.

For making retrovirus, Plate-E cells were transfected with retroviral vectors and pEco (the plasmid containing packaging sequences) using LipoD293 transfection reagent (SignaGen Laboratories, Rockville, MD, USA).

The resulting plasmids containing optimal packaging sequences were designated pCMV-5′LTR-psi-Luc and pCMV-5′LTR-psi-Luc-3′UTR.

In some cases, genomic NAs are preferentially packaged over others with equivalent charge (Borodavka et al., 2012) due to virus-specific packaging sequences (Bunka et al., 2011; Borodavka et al., 2012).

For instance, linking the MS2 packaging sequence to the human growth hormone (hGH) mRNA enabled the packaging of this particular mRNA in MS2 VLPs.

We show that MS2 can be produced as virus-like particles (VLPs) in Saccharomyces cerevisiae and is able to package functional heterologous mRNAs, provided that these mRNAs contain the MS2 packaging sequence.

The mRNA transcribed from these vectors contained optimal MLV packaging sequence (+206; +1106, Genbank: AF033811) [ 8, 11].

The reason for this is unknown but presumably reflects a more favorable context for CMV-driven expression proximal the 3' LTR compared to near the packaging sequence.

B204 binds DNA unspecifically [ 7] which suggests that it does not bind to a specific packaging sequence on the STIV2 genome.