Sentence examples for packaging limit from inspiring English sources

However, these vectors are limited by their packaging limit, in which the coding sequences of Sp-Cas9, the most well-used Cas9 and its sgRNA are almost reaching (Cox et al., 2015; Maeder and Gersbach, 2016).

We designed the Ad vector genome by insertion of a transgene expression cassette into the E3 region, such that homologous recombination between the Ad vector DNA and 293 cell-derived Ad E1 DNA would produce an Ad vector genome that exceeds in the size of the packaging limit.

The packaging limit of AAV is 5.2 kb [15] and accommodation of large transgenes requires minimizing the size of cis elements in the expression cassette.

The enormous size of the full-length dystrophin gene poses one of the biggest challenges for gene therapy because it exceeds the packaging limit of most viral vectors.

AAV-mediated gene therapy presents a desirable treatment strategy for multiple diseases; however, it is hindered by the restrictive 4.7 kb packaging limit of the AAV virion.

One drawback is that it cannot fit into the 5-kb packaging limit of AAV, the most efficient muscle gene-transfer vector.

The legislation would require that tobacco products be sold in plain green packaging, limiting the brand recognition enjoyed by global tobacco names like Marlboro and Camel.

The coding sequence of USH2A is >18 kb, and the packaging limits of AAV and EIAV vectors are about 3 kb and 10 kb, respectively.

200 μl of the A. tumefaciens– Z. tritici mixtures were plated onto nitrocellulose filters (AA packaging limited, Preston, UK) placed on AIM agar plates supplemented with 200 μM acetosyringone and grown at 18 °C for 3 days.

Briefly, A. tumefaciens cultures (OD595 = 0.25 0.30) carrying the appropriate vector and a Z. tritici cell suspension (1 × 10 cells/mL in sterile water) were mixed in a ratio of 3 1 and 400 μL of this mixture was spread on nitrocellulose membranes (A.A. Packaging Limited, Preston, UK) placed on IM agar.

When (alpha_{i} > 1), customer (i) pays extra charges to compensate for the consumption that exceeds the package limit.