Sentence examples for packaging extracts from inspiring English sources

High efficiency, restriction-deficient in vitro packaging extracts for bacteriophage lambda DNA using a new E.coli lysogen.

Resulting fosmids were packaged using MaxPlax™ Lambda Packaging Extracts and transfected into Epi300-T1R E. coli cells following protocols for the CopyControl™ Fosmid Library Production kit (Epicentre).

The λ transgenic shuttle vector recovery was performed as described by Stratagene's Transpack Packaging Extracts producing packaged DNA samples as λ-phages, with adaptations for cauda spermatozoa; 8-15 µl DNA was packaged, and when necessary incubation was prolonged (with up to 2 h) and/or additional packaging extract was added.

Ligated DNA was packaged into phage particles using lambda packaging extracts (Stratagene).

Each ligation was packaged with the Packagene Lambda DNA Packaging Extracts (Promega, USA).

The ligated mixture was then packaged into lambda phages using MaxPlax Lambda Packaging Extracts (Epicentre, Madison, U.S.A).

The ligated DNA products were then in vitro packaged using Gigapack III packaging extract (Stratagene, USA).

Subsequent packaging was carried out using Gigapack III gold packaging extract (Stratagene, USA).

Gigapack III Gold packaging extract (Agilent Technologies) was used for packaging the DNA according to the manufacturer's instructions.

This vector has a Murine Stem Cell Virus (MSCV) backbone combined with packaging extract for mammalian cell infection, a PGK- Puro selection for transfection stability in mammalian cells and a chloramphenicol/kanamycin bacterial selection marker.

Briefly, Sfi-I digested and purified cDNAs were directionally ligated and packaged into the Lamda TrplEx2 vector using Gigapack III Gold Packaging Extract (Stratagene).