Exact(1)
A transfection mix was prepared with either 5, 10, or 15 μg of DNA of the STEMCCA vector or control GFP lentiviral vectors (EF1α-GFP; both kindly provided by Dr Gustavo Mostoslavsky), packaging cassette (REV and Gag/Pol, 10 μg) and the VSV-G (5μg) envelope expression cassette, respectively.
Similar(59)
(The Christie's catalog uses a British spelling of dispatched. ) The version for sale through Christie's includes "the original packaging, manuals, cassette interface and basic tape, early documentation and provenance, and a commercially rare letter from Steve Jobs".
In addition, my family sent care packages, letters and cassette tapes recorded with long, rambling messages cataloging everyday moments.
For the AAV vector preparations used in the competition experiments physical titres were determined by PCR quantification using TaqMan with a probe annealing on the bovine growth hormone (BGH) polyadenilation signal, included in the EGFP-expressing cassette packaged in the AAV vectors.
The cassette's packaging featured a knife-bearing angel, a photo from the Holocaust of a cadaver being pushed into a crematorium, and a reproduction of the Rylands Papyrus, one of the oldest surviving fragments of the Gospels.
When the conventional retroviral vector was transfected into packaging cells, the expression cassette drove strong GFP expression, but no infectious retrovirus was produced.
Because efficient amplification of adenoviral vectors containing an adenoviral DNA replication-targeting amiRNA cassette in packaging cells requires the shutdown of amiRNA expression in these cells, amiRNA expression in our system is under the control of a tetracycline repressor/operator system.
He also remastered the song, selling it by mail order, along with an instrumental version, in a limited edition package consisting of a compact disc and cassette tape — the package could be purchased at a cost of approximately $30.
We were given lunch, listened to the new work, and presented with a package that included a cassette, an LP and a CD.
Within the framework of the Work Package DIV 1 – "Divertor Cassette Design and Integration" of the EUROfusion action, a research campaign has been jointly carried out by ENEA and University of Palermo to investigate the thermal-hydraulic performances of the DEMO divertor cassette cooling system.
The shop was much more than a place to buy a limited-edition cassette tape packaged in human hair, it was a landmark location for noise enthusiasts.
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