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The filtering procedure made use of the packages signal [ 50] and seewave [ 51].
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In his paper, he describes how he took the packaging signal from segment eight, and put it on segment four.
Unexpectedly, a preferential use of the packaging signal for recombination was observed, as compared to the IRES.
The interpolated values have been obtained using the command pchip of the R package Signal, which uses the methodology described by Fritsch and Carlson (1980).
Because of the retroviral packaging signal, the ribozyme would be expected to colocalize with the lacZ-containing viral genomic RNA but not with the lacZ messenger RNA.
The HSV amplicon vector incorporates features of HSV-1, including a 150 kb transgene capacity, a viral origin of DNA replication and packaging signal, and virion proteins.
In many DNA viruses, genome packaging is initiated by the small subunit of the packaging terminase, which specifically binds to the packaging signal on viral DNA and directs assembly of the terminase holoenzyme.
The Ψ-RNA packaging signal of the human immunodeficiency virus type-1 (HIV-1) genome contains a 35 nucleotide stem-loop, termed SL1, which is important for efficient genome packaging during virus assembly and for reverse transcription during infectivity.
Our findings identify a 159-residue RNA packaging signal that possesses dimerization and NC binding properties similar to those of the intact 5′-L and contains elements required for efficient RNA packaging.
The distinctive binding mode of NC to SL2 and SL3 stem loops of the HIV-1 genomic RNA packaging signal is studied on the basis of protein side-chain contributions to the electrostatic binding energies.
Southern blotting with a probe for the MLV packaging signal was described earlier [7].
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