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Binding windows were predicted for all candidate clusters of MXEs using the SeqAn [ 33] and the ViennaRNA [ 34] packages, and, subsequently, also for the available exon-intron gene structures from the related species as obtained in the cross-species searches.
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Thus, the rescue titer is a measure of the ability of a given vector combination to co-package and subsequently generate a provirus.
For SPM analysis, the metabolic images were spatially normalized using the template provided in the SPM 99 package and subsequently smoothed with a 16 mm isotropic Gaussian kernel.
To our knowledge, the value of SPIO for targeted imaging lies in the fact that the SPIO surface can be packaged and subsequently combined with appropriate targeting ligands.
Finally, reference sequences containing loci to be examined were created against which short reads were mapped using the Burrows–Wheeler Alignment (bwa) software package and subsequently viewed using Tablet (Li and Durbin, 2009, Milne et al., 2013).
To this end, we invoke in R the gMCP package and subsequently call the GUI with > library gMCP) > graphGUI Different buttons are available in the icon panel of the GUI to create a new graph.
If applicable, band densities corresponding to free, singly and doubly bound nucleosomes were quantified using ImageJ software package and subsequently fitted together to a 2 1 binding model using in-house written MatLAB routine (MATLAB version 7.13.0, The MathWorks Inc., Natick, MA).
Specific transporters expressed on the vesicle membrane determine the transmitter cargo that will be packaged, carried, and subsequently released following fusion with the plasma membrane.
The ligated DNA was packaged using the lambda packaging extract supplied and subsequently transformed into Escherichia coli EPI300-T1R.
13 Given their reliance on branding to attract new and reassure existing users, tobacco companies strongly resisted standardised packaging and have subsequently opposed its introduction in every jurisdiction where it has been mooted.
IKB received a bailout package 10 days later and subsequently got as much as 12 billion euros in guarantees from Germany's bank-rescue fund.
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