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Nearly identical patterns were found for the other software packages (Additional file 3: Figure S1), so the effects are not caused by the statistical algorithm used to identify differentially expressed genes.
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At that time, Best Buy struck a deal to package additional material, like photographs and archival interviews with the band members, with each CD.
The analysis was made using the R 3.0.1 statistical package (Additional file 3).
Network properties and communities were computed by using the igraph R package (Additional file 2).
But, we noted many upregulated genes that were enriched in gene sets involved in telomere maintenance, telomere extension and telomere ends packaging (Additional file 1: Table S3A & B).
The ORFs were predicted by the "Getorf" program from the EMBOSS software package (Additional file 4; http://emboss.sourceforge.net/apps/cvs/emboss/apps/getorf.html).html
A GO term enrichment analysis of the three polysaccharide-induced gene expression patterns compared to the SGEP was performed with the topGO R package (Additional file 12) [ 47].
To visualize the pattern of differentially expressed genes in the samples (at least twofold), plotSmear plots were generated using the edgeR package (Additional file 1: Figure S3).
Furthermore, we performed a prediction of type III secreted proteins encoded in the genome using the EFFECTIVET3[ 64] software package (Additional file 4, Table S3).
ISOpure is freely available as a MATLAB package (Additional File 1) that can also be run in the open-source Octave environment with a small number of modifications; the latest version is maintained here [ 36].
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