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The package was collected in a police car and taken to the station.
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A total of 57 clam packages (30 60 clams per package) were collected from supermarkets or fish markets from 6 different areas in western Japan from December 8 , 2005 to September 6, 2006.
Howard Yaruss, founder of the Application Project Inc., the group behind the website, told the Chronicle of Higher Education that application packages are collected directly from admitted students in exchange for a stipend of up to $100.
Three of 4 sapovirus-positive clam packages were collected from different areas and at different times.
Media containing the packaged virus was collected on day 2 after transfection, fresh media was added, and the viral harvest was collected again on day 3 after transfection.
Supernatant containing packaged lentivirus was collected and passed through 0.45 μm filters after 48 h of transfection.
The packaged virus was collected at 24 and 48 h post-transfection and concentrated using ultracentrifugation (70,000 g, 2 h, 15°C).
Two days after transfection, the medium containing newly packaged retrovirus was collected and filtered through a 0.45- μm pore size filter.
In total, one tonne of equipment was collected, packaged and boxed with labels within 5 days (Fig. 1c).
For lentivirus generation, the generated constructs were transfected into 293 T cells together with enveloped and packaging plasmids and supernatant was collected 48 h post transfection.
The salt content (g/100 g) was collected from product packaging and nutrient information panels of cheeses available in the top seven retailers.
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