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The results of the LD analysis were used to reconstruct haplotypes (R package GAP) of the TGFB2 and HMOX1 genes and test whether the most frequent haplotypes (>5%) were associated to CM correcting statistical significance using permutation tests.
In a third step, we used the gene counting method available in the R package GAP to reconstruct haplotypes composed with multiple associated SNPs within an LD block [56].
Genomic coverage was visualized as Manhattan plot via the mhtplot function from the R package gap.
The complete genome sequence was finished by using the Staden package (GAP v4.8b1) [ 44].
Pairwise sequence comparison was carried out using the Smith and Waterman local algorithm implemented in the water program of the EMBOSS package (gap opening penalty 10, gap extension penalty 0.5, EDNAFULL matrix).
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The Manhattan plot, showing genome-wide P-values of GWAS, was produced using R 2.13.2 software with the "gap" package (Zhao 2007).
Sequence identity was determined by pairwise alignment using the DNAMAN software package (optimal alignment; gap open penalty 10.0, gap extension penalty 5.0).
A Manhattan plot of the p-value results from the GWAS was produced using R 2.13.2 software [ 52] with the "gap" package [ 53].
Its nucleus is begin{aligned} mathcal{N } =, { 1, a, b, c }, end{aligned}and the only non-trivial relators of length (le 3) among elements of (mathcal{N }) are (a^2 = b^2 = c^2 = 1) (this can best be checked with the GAP package (http://www.gap-system.org/Packages/automgrp.html).org/Packages/automgrp.html
The customProDB package fills this gap by providing an efficient tool to generate customized protein databases using expression and variation information available from NGS data.
Genome-wide p-value against SNP physical position (by chromosome) genome plots were generated using the gap package in R, version 2.7.2 (Zhao 2007).
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