Sentence examples for package cells from inspiring English sources

Complete clinical-grade viral particles were produced using transfection package cells with a four-plasmid system.

In this scenario, a fibrillar FN network is absolutely crucial to package cells into a somite.

The plasmid constructs, pS2-maspin and pS2-blank vector were transfected into 293T package cells to produce infective viral particles.

The expression vectors were transfected into 293FT package cells (R70007, Invitrogen, USA) using a retrovirus packaging kit (D6161, Takara, Japan) to produce recombinant viruses.

For transduction of these cells, the retroviral vector pBABE-Gαq-YFP was transfected into Phoenix-Eco package cells, and the supernatant, containing viral particles, was harvested 48 hours after infection.

Construction of package cell PA317/TK has been previously described [ 7].

Then the pellet was washed by 5 package cell-volume buffer A without NP-40.

For retrovirus production, the viral polyproteins (gag, pol, and env) were transfected into 293FT packaging cells.

The transfection mix was transferred to the packaging cells.

PA317 amphotropic packaging cell line was infected with the recombinant retroviral vector, and the supernatants from the packaging cells were added to the F3 hNSCs.

HEK293FT cells were used as packaging cells, and virus production was as previously described [42].