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Means and 95%% confidence intervals of individual buds (n = 3) were calculated using R R Development Core Teamm, 2014) and graphics were compiled using the latticeExtra package and functions within (Sarkar and Andrews, 2013).

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Because the data are directly returned into R, all packages and functions in Bioconductor (24) can be integrated with the system.

The user can also use a number of existing bioconductor packages and functions to perform quality control on their data, and can check the adequacy of reference genes visually.

Function pois.exact in package epitools and function midPci in package PropCIs, both contained in R environment for statistical computing, 22 were used in these computations.

Variation in composition among treatments was assessed with Principal coordinates analysis (PCO), using the cmdscale function in the R base package and wascores function in vegan.

Mediation analysis was performed with the "lavaan" package and "sem" function (ver. 0.5 18) (Rosseel 2012) in R (ver. 3.2.1).

To estimate cell-type proportions, we used the R minfi package and estimateCellCounts function [ 123, 124].

We used a standard singular value decomposition method from the 'pcaMethods' R package and lm function for the linear least squares.

For each isolate's time course, only genes having at least 80% of data present were extracted and any missing data values were imputed by KNN (Kth nearest neighbor) method using R version 2.10.1 package and impute function.

BRT modelling and statistical tests were performed with the software R (Core Team R 2012), including the gbm package (Ridgeway 2014) and functions written by Elith et al. (2008).

Still others are used to provide packaging and infectivity functions for non-phage DNA.

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