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Remarkably, A549 cells treated with 2.5 nM SAG showed a more pronounced upregulation of TP53 target genes compared to cells treated with 4 nM PAC (Table 4).
Interestingly, GO analysis revealed that genes involved in DNA damage response and repair pathways such as the human DNA Polymerase epsilon (POLE) [26], XRCC6 and XRCC5 [27], were found to be upregulated after treatment of A549 cells with 40 nM SAG or PAC (Table 3).
Genes involved in G2/M phase transition and mitosis, such as Cyclin A, Cyclin B, Nek2A and Securin [23]; [24] and genes such as BUB1, BUBR1 and CDC20, which are components of the spindle assembly checkpoint (SAC) [25], were upregulated after treatment with high concentration of SAG or PAC (Table 2).
STH15 and StBEL5 exhibited the greatest levels of accumulation in PAC (Table 8).
Pumilio was selected because of its relative abundance in PAC (Table 5), its functional relevance, and its widespread role in RNA metabolism [ 68].
In addition, there was a proportional reduction in the number of cells, the percent of spreading, and cell viability with the increased dose of PAC (Table 1).
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Few of these are highly expressed exemplified by the fact that there are only ten petiole-PAC unique genes with greater than 500 reads (Table 3), and only 26 in stem-PAC (Table 4).
A rice jmjC-domain protein functions in controlling suppression of flowering [ 46].>> Gene ontology categories analyzed for the DE genes were also considered for the uniquely expressed genes in petiole-PAC and stem-PAC (Table 1, Group 2).
With 10 reads as a mean threshold, approximately 11 k of the genes are expressed in both petiole-PAC and stem-PAC (Table 1, whole genome column, Group 2).
The 2 million high-confidence PATs define approximately 42,600 sense-oriented PACs (Table 2).
All six KN1-like RNAs contain PTB motifs and three of the six are relatively abundant in PACs (Table 7).
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