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Although some solvents reduce the productivity, an enhancement of the PAC production from 1.8 mM in the buffer control to 3.1 mM is also possible, for example with 5 vol% MTHF.
We demonstrated the improvement of penicillin acylase (PAC) production by optimization of the host/vector system using genetic engineering strategies.
The other solvents which enhance the PAC production are DMSO, dioxane, EtOAc and iProp (Supporting Information, Figure S5).
Specific mutagenic activity peaked in a temperature range of 1300K to 1500K and generally appeared at higher temperatures and longer residence times than peak PAC production.
However, in contrast to PAC production by ApPDCE469G, we observed no significant shifts towards the R-enantiomer in the presence of the cosolvents (Supporting Information, Figure S3).
The second obvious trend of the organic cosolvents is a shift of the chemoselectivity towards the smaller 2-hydroxy ketone in most cases, except for the PAC production catalyzed by LlKdcA and PpBFDH281A, where HPP is reduced while both PAC and acetoin rpd values are increased.
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It already controls 22% of Japan's weapons business and will make antimissile systems under license from Lockheed Martin in the first foreign PAC-3 production.
This is the first report of enhancing (R -PAC pR -PAC in yeast by recombinant technology with caproducty of commercinl production.
For insect cell production, the pAc-κ-Fc vector from PROGEN Biotechnik (Heidelberg, Germany) was used.
The combination of mutant Pdc1 in pdc null background was successfully evaluated for production of (R -PAC at industR -PACcate.
The aim of the study was to increase production of (R -PAC by alteR -PACarbyligalteringtivity of Pdcarboligationmyces cerevisiactivity
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