Sentence examples for pac expression from inspiring English sources

It was contrived with the purposes of (i) providing sufficient osmolarity and buffering capacity, (ii) minimizing ionic species to facilitate the binding of extracellular proteins to anion-exchange media, and (iii) enhancing PAC expression level and secretion efficiency.

Neither PAC expression alone (without stimulation by blue light), nor light exposure to Or83b-/ flies resulted in significant changes in spiking activity of the neurons (Figure S5).

In ab2A, ab2B, ab3A and ab3B neurons, the light induced increase in spike rates was significantly higher than in wt flies, showing that the increase in spike rates upon blue light exposure depends on PAC expression (Figure 6E).

Additionally, in order to achieve good levels of pac expression, we cloned the IRES of the encephalomyocarditis virus between the EGFP and pac genes which allowed the bicistronic mRNA transcript to be translated from the 5' end and from the IRES sequence, so that the pac gene was also under the control of the strong synthetic late promoter.

Lactose concentration measures during cultivation B2 showed that, despite continuous hydrolysis of lactose, it was possible to maintain a residual inducer concentration capable of promoting pac gene expression throughout the induction phase.

ACN supplementation inhibited adenosine diphosphate (ADP -induced plADP -inducedation-related conformational change and degranulation by reducing plateletpression by 12% activation-relatedression by 9% respeconformational

Lentivirus system transfection was performed using Lenti-Pac HIV Expression Packaging Kit (GeneCopoeia, Rockville, MD, USA) according to the manufacturer's instructions.

Platelet activation was further evaluated by detecting P-selectin (CD-62p) and PAC-1 expression on the exposed platelets using flow cytometry (BD Calibur) as previously described.

Lenti-Pac HIV Expression Packaging mix (GeneCopoeia™), pLV-miR-137 or pLV-miR-NC were cotransfected into the 293Ta cells using EndoFectin Lenti transfection reagent and following the manufacturer's instructions.

Packaging of the miR-29b constructs in pseudoviral particles was performed in 293Ta cells using the Lenti-Pac FIV Expression Packaging Kit (FPK-LvTR-20), according to the manufacturer's instructions (Genecopoeia, Rockville, MD, USA).

Pseudo lentiviral particles for TK1 overexpression and the control were produced with Lv-TK1 or the control plasmid and Lenti-Pac HIV expression packaging kit (GeneCopoeia, USA) on 293T cells according to manufacturer's protocol.