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During early FPLC-separation steps (e.g. first Mono-Q separation), the elution profiles showed up to four heme peaks with veratryl alcohol oxidizing activity; we focused our purification efforts always on the most active fraction (i.e. that with the highest specific activity).
Ag enhances oxidizing activity of copper, and BMNP Ag/Cu have higher surface reactivity as compared to their constituents [31].
Diminishment in catechol oxidizing activity of A. bisporus PPO in the presence of NaN3 was observed along with a slight reddish colouration of assay mixtures.
In contrast, the presence of 2 mM kojic acid and arbutin (tyrosinase inhibitors) gave only negligible inhibitory effects on A. chroococcum PO (3% inhibition) as well as fungal PO (1% inhibition), whereas catechol oxidizing activity of A. bisporus PPO was reduced to 50% relative activity.
We demonstrated that the low increase in specific activity after Ni-NTA is due to the intrinsic aldehyde oxidizing activity of E. coli enzymes of the xanthine oxidase family present in the crude extract (data not shown).
Because Mo-dinitrogenase complex activity has exceedingly slow in vivo turnover (<10 sec−1), any directly coupled Hyq endo-hydrogenase H2 oxidizing activity might operate at correspondingly very slow rates in vivo.
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Among them, the NAD+-dependent GDH of Hansenula polymorpha showed the highest glycerol-oxidizing activity.
Finally, the bioleaching tests showed that strain Cutipay resistance enhances chalcopyrite bioleaching at 50°C in the presence of chloride, based on its fast iron-oxidizing activity compared to the chloride sensitive strain Sulfobacillus acidophilus DSM 10332.
Based on the kinetic quantification of chemical oxidation of Fe(II), it thus becomes evident that Pseudogulbenkiania sp. MAI-1 can directly oxidize Fe(II), establishing the organism as a novel neutrophilic nitrate-dependent chemotroph with unambiguous biological Fe(II -oxidizing actII -oxidizing
The variants obtained largely maintain the acidic stability and strong Mn2+-oxidizing activity of the parent enzyme, and the ability to oxidize veratryl alcohol and Reactive Black 5 (two simple VP substrates) was introduced.
As studied in archetypal aerobic bacteria such as Ralstonia eutropha, orthodox respiratory hydrogenase is a heterodimeric protein comprising a bimetallic Ni,Fe-catalytic subunit and a 4Fe-4S-center subunit, which complex with an integral-membrane b-type cytochrome, linking Ni,Fe-hydrogenase H2-oxidizing activity to cellular respiration and oxidative phosphorylation [12].
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