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But basically it's there for viewers to stare at the cars' absurd lit-up undercarriages, diminished seating capacity and nitrous-oxide kits.
The levels of total nitrite in lavage fluids were measured with Nitric Oxide Assay kits (Calbiochem®; Biosciences Inc, La Jolla, CA, USA).
Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), prostaglandin E2 (PGE2) and nitric oxide (NO) kits were purchased from R&D systems Inc , USA
Red blood cell lysis buffer, kaumas blue, cell lysis buffer for western and IP, Nitric Oxide Assay Kit, Total Superoxide Dismutase Assay Kit with WST-8, Lipid Peroxidation MDA Assay Kit, and Reactive Oxygen Species Assay Kit were purchased from Beyotime Biotechnology (Shanghai, China).
Total NO was determined in the conditioned concentrated supernatants by quantifying nitrite, the stable end product of NO oxidation, spectrophotometrically using a colorimetric nonenzymatic nitric oxide assay kit (Oxford Biomedical Research, Oxford, MI, USA) as described previously [ 9]. Cell growth was determined by total live cell counts using trypan blue exclusion.
High molecular weight proteins, which would otherwise interfere with the NO assay were removed from the lysates with Ultrafree Biomax 10 kDa columns (Millipore) by centrifugation at 11,000 rpm for 15 min. Total NO in the sample was quantified using the Endogen Total Nitric Oxide Assay Kit (Pierce) according to the manufacturer's protocol.
The Griess reagent nitric oxide assay kit was purchased from Beyotime Biotech (Jiangsu, China).
NO levels were measured using the OxiSelect Intracellular Nitric Oxide Assay Kit (Cell Biolabs, Inc).
NO production was estimated from nitrite levels using a Nitric Oxide Assay Kit (Pierce).
Serum NO level were measured by QuantiChrom™ Nitric Oxide Assay Kit (D2NO 100) obtained from i-DNA Biotechnology Sdn.
Total nitric oxide assay kit (Thermo Scientific, Rockford, IL) was used for the assessment effects of IL-1β induced nitric oxide production in culture supernatants.
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