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Chemiluminescence measurements of nitrogen oxides were performed using a Bo 280i Nitric Oxide Analyzer (NOA; Sievers, Boulder, CO, USA) with output data analyzed using either the LIQUID program or OriginPro 8.0.
N02¯ and N03¯ determination was performed using the Sievers Nitric Oxide Analyzer.
Plasma nitric oxide metabolite content was then measured following ethanol-induced protein precipitation and centrifugation using a vanadium III) assay to reduce nitrate, S-nitrosothiol and nitrite to nitric oxide that was then detected in a helium driven, ozone/I3-based chemiluminescent nitric oxide analyzer (Sievers, Model 280 NO analyzer, Boulder, CO, USA).
Fraction of exhaled nitric oxide (FENO) was measured with an online measurement of resting values using a restricted exhaled breath protocol (NOA 280i Nitric Oxide Analyzer, Accurate NO Breath Kit, Thermal Mass Flowmeter, NO Analysis software Version 3.21, Sievers Instruments, Boulder, CO).
Exhaled breath was measured with a chemiluminescent nitrogen oxide analyzer (model 200A; API, San Diego, CA).
The chemiluminescence was detected using a nitric oxide analyzer (NOA280, Sievers Instruments, Boulder, CO, USA) [ 18].
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Arterial NOx (nitrite + nitrate) and RBC O2-dependent ATP efflux were measured using a nitric oxide (NO) analyzer and gas exchanger, respectively.
We measured exhaled nitric oxide with a chemiluminescence analyzer (Sievers Model 280 NOA; Sievers Instruments Inc., Boulder, CO, USA) according to American Thoracic Society recommendations (American Thoracic Society 1999).
The black materials, which were thought to be manganese oxide precipitates, were analyzed non-destructively on-site using a pXRF analyzer (Delta Premium, Innov-X Systems, Waltham, MA, USA) with an X-ray tube with a Rh target (4 W).
We continuously monitored the air in the exposure chamber for temperature, humidity, nitrogen oxides [chemiluminescence NO-NO2 NOx analyzer; Thermo Environmental Instruments (TEI), Franklin, MA, USA], carbon monoxide (gas filter correlation CO analyzer; TEI) sulfur dioxide (pulsed fluorescence SO2 analyzer; TEI), and ozone (absorption ozone analyzers model 8810; Monitor Labs, San Diego, CA, USA).
Limestone elemental analysis was conducted using a Varian 725 ICP-OES for major elements (except SO3, which was determined using a LECO sulfur analyzer) and presented in oxide form.
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