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Since it has been reported that phoP mutants are more susceptible to oxidative killing in vitro [22], we asked whether phoP mutants would be more virulent in CGD mice, and they were not.
Brucella htrA mutants have been described as being temperature sensitive, sensitive to oxidative killing in vitro, sensitive to killing by macrophages, and attenuated in both mice and ruminants [17], [27], [28].
Consequently, human Mac-1 plays a role in regulating leukocyte migration, phagocytosis and oxidative killing.
In addition, we found phagocytosis of pneumococci delayed and oxidative killing reduced in TLR2-/ compared to wt granulocytes in vitro (14).
With the oxidative killing ability of neutrophils hindered, diabetics may not be able to clear pathogens as well as non-diabetics [ 1, 6, 7, 31].
Superoxide, for oxidative killing of pathogens, produced by leukocytes is dependent on oxygen levels.
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Furthermore, growth hormone primes neutrophils for production of lysosomal enzymes and superoxide anions, supports survival of memory T cells, increases immunoglobulin secretion of B cells, and stimulates thymulin secretion by thymic epithelial cells, natural killer cell activity, phagocytosis, oxidative burst, and killing capacity of neutrophils or macrophages [ 120].
In particular, their promotability by oxidants and their sensitivity to killing and oxidative macromolecular damage are being measured.
Kolotila et al., 1990, reported that opaque cells were more susceptible than white cells to killing by oxidative stress and by neutrophils [11].
H2O2 exposure did not increase the rate of translation of Rpl22b, and only loss of the gene for Rpl22a rendered the cells sensitive to killing by oxidative stress.
These results suggest that inhibition of oxidative stress protects islets from glucotoxicity, but deletion of either Bim or Puma on their own does not reduce the residual oxidative stress-mediated cell killing in NAC-treated islets.
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