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We tested this production in the Raw264.7 cells in parallel with oxidative data.
Indeed, it must be taken into account that although the oxidative data shown were obtained at baseline, the diagnoses were only confirmed during the follow-up.
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Normality of oxidative stress data was assessed by Shapiro-Wilk test; data were normally distributed.
Using DEHP oxidative metabolite data, the estimated DEHP exposures are about twice those calculated from MEHP data (Tables 1, 2, 4).
The oxidative stress data for the aged G/Z- and Y-TZP-treated cells showed a significant increase at 72 h.
Oxidative stress data for the aged G/Z- and Y-TZP-treated cells showed a significant increase at 72 h (P < 0.00001, Fig. 1b).
The oxidative stress data were analyzed with nominal logistic regression, modeling the log odds (mortality/survivorship).
Both assays supported the assumption that the light conditions used were indeed causing oxidative stress (data not shown).
P. fluorescens and HepG2 cells exposed to metal toxicants and ROS-producing molecules accumulated succinate in the media, a biomarker for oxidative stress (data not shown) [13].
The response elicited by the ΔphoP mutant was very similar to that induced by the WT strain, i.e., no pH changes nor oxidative response (data not shown and Figure 6D).
These include ROS and the transcriptional activity of NRF2, which are both predicted increased in each of the oxidative stress data sets (Hyperoxia and HOCl).
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