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The thermo-oxidation of XLPE substrate has been assessed by isothermal differential scanning calorimetry (DSC) and infrared analysis (FTIR) to determine the oxidative induction time (DSC-OIT) and the oxidation index, respectively.
From the above studies we have concluded the following: First, post-electrophoretic identification of oxidized proteins using 2,4-DNP is possible; Secondly, post-electrophoretic identification of specifically oxidized proteins can be quantitative and the specific oxidation index can be determined; Thirdly, colloidal gold is preferred as the protein stain.
The results of our study support that second-generation sequentially annealed HXLPE has improved oxidative properties (as seen by a reduction in maximum oxidation index) compared with first-generation annealed HXLPE.
Oxidation index (OI) showed similar trends to the surface penetration depth.
The mechanical properties and extent of oxidation of all inserts were characterized using a standard small punch test and measurement of the oxidation index.
Within the studied variable range, microfluidization does not increase the P oxidation index, nor does it significantly alter the P concentration.
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Oxidation indices of sequentially annealed liners were positively correlated with implantation time and shelf life.
However, sequentially annealed HXLPE had lower oxidation indices than annealed HXLPE in all measured locations.
For the sequentially annealed cohort, implantation time was positively correlated with oxidation indices at all measured locations (Rho = 0.21 0.43; p ≤ 0.02).
The rim had higher oxidation indices than the bearing surface (mean OI difference = 0.2; p = 0.01) and the backside surface (mean OI difference = 0.2; p = 0.03).
For the annealed cohort, the regional variations are similar in pattern but were more pronounced with mean differences in oxidation indices of approximately 2.5.
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