The coding regions affected by frame-shifts owing to microsatellite indels have undergone changes indicative of gene fission/fusion, premature termination and length variation.
We restricted analysis to nucleotide variation within C. remanei and to divergence between species, owing to the few indels detected within the smaller C. sp. 23 population sample (table 1).
Definitive proof that the nature of the cuts provides advantages for precision genome editing for clinical applications remains elusive and many researchers in the field favor inducing single-stranded breaks owing to the reduced risk of creating indels.
However, owing to differences in allele frequencies, the indel had only a low correlation (r) with many of the SNPs (Fig. 4).
However, in coding regions, 75.4% of LS insertions and 60.1% of LS deletions are spurious, owing to the substantially reduced rates of true indels in these regions.
PCR products were effectively separated and easily scored after 30 min of electrophoresis on 6 % polyacrylamide gels owing to our use of short amplicons and indel to amplicon size ratios larger than 3%%.
InDels and SNPs are the markers of choice for high-resolution genetic mapping and association studies owing to their abundance and distribution throughout the genome [ 22, 23].
Venezuela and Nigeria are unreliable, owing to civil strife.
Turnout was low, owing to the snow.
