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To assess protein expression during a reproductive cycle, female mosquitoes were bloodfed and tissue samples were collected at various time points (0 (non-fed), 3, 6, 12, 24, 36, 48, and 72 hr (post oviposition)) from fully engorged females.
The gene encodes a member of a family of putative vesicle cargo receptor proteins that may mediate the transmission of positive signals for oviposition from the central and ventral nerve cord [ 62].
Four clutches of green turtle eggs were collected immediately after oviposition from females on Heron Island, (23°26′S, 151°51′E), Southern Great Barrier reef (GBR) in December 2013 and transported to a laboratory at the University of Queensland in Brisbane where eggs were incubated at a constant temperature of 28°C buried in vermiculite with a water potential of ∼100 kPa.
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Two unidirectional, normalized cDNA libraries were constructed from embryos ranging in age from 30 min to 36 hr after oviposition and from adult male and female heads of flies ranging from 30 min to 8 days after emergence.
Several studies have shown that the selections of gravid females to potential oviposition sites from a distance were mediated by volatile signals, however, the means by which the sensory cues from non-volatile chemicals affected the insect behavior were still a controversial subject.
We isolated the oviposition pheromone from the Southern house mosquito, analyzed the extract by GC with a chiral column and confirmed that Cx. p. quinquefasciatus produces (5R,6S -MOP (Fig. 7C) thus ruling out possible geographical or other variations.
As oviposition ranged from EGK-VI to EGK-VIII, we examined these stages.
The oviposition plate from control and experimental condos was made 24 hr later.
In other Galloanserae, oviposition ranges from EGK-VII (Turkey [ Gupta and Bakst, 1993] and Duck [ Sellier et al., 2006]) to EGK-XI (the quail [ Stepinska and Olszanska, 1983]).
The oviposition plate from each fly condo was replaced 24 hr after the start of the experiment, and the second plate was removed 48 hr after the start of the experiment.
Furthermore, we recorded the time in days from oviposition until egression of larvae from their host and from oviposition to the emergence of the first and of the last adult wasp.
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