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Due to overt effects observed within 18 min at all test concentrations, the study was terminated and all fish were humanely euthanized to avoid additional stress.
To investigate if we could demonstrate such overt effects of the mutations, we turned towards the adult rescuants.
Together, these results suggest that the absence of dystonin has no overt effects on the morphological and histological features of the heart, at least in young postnatal mice.
We have ruled out overt effects on temperature-sensitivity, cell growth, cell morphology, protein localization, and polarization kinetics in the presence and absence of actin.
Thus, a 16 g bat should require 1.03 ml of ethanol sugar water to achieve a BAC of 0.11 g 100 ml−1 [41], [41], which we expected would produce overt effects of intoxication.
Enzymatic activity of CK-B was indispensable since cyclocreatine completely blocked the gain in migration capacity in both CK-B expressing cell pools (Figure 6C and D, Video S3) without overt effects on viability or proliferation rate (Figure S1).
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However, deletion of Cebpd in mice had no overt effect on ovarian physiology and reproductive function.
Nocodazole had no overt effect on mitochondrial location or morphology in hDMPK C-expressing cells.
Consistent with previous finding from chronic alcohol administration study [15], ADH transgene accentuated the acute ethanol challenge-induced cardiomyocyte mechanical dysfunctions without eliciting any overt effect by itself (Fig. 2).
To further confirm the basis of the phenotype, we examined the expression of markers of prehypertrophic and hypertrophic chondrocytes in transverse sections through the limbs and vertebrae at times before an overt effect on mineralization was apparent.
Comparison of the actin and microtubule cytoskeletons in cells with YFP-hDMPK A or C expression did not reveal any overt effect on structural integrity (Figure 2A and 2B).
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