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Comparison with previous radiological examinations is required to detect subtle or overt changes in the location and position of the pump and catheter.
Somewhat surprisingly we found no overt changes in the blastoderm stage expression of several different segmentation genes in embryos that lack maternal NELF-A (see below).
Previous reports only presented vague systemic inflammatory infiltration without overt changes in the intestinal epithelium (Fleming et al., 2010; Oehlers et al., 2011; Oehlers et al., 2012).
In absence of Tcf7l2 function, we see no overt changes in the timing or patterns of neurogenesis in the dHb or in the numbers of neurons generated.
We observed no overt changes in the pattern of total cellular VEGFR2 expression over time after VEGF stimulation when comparing the four genotypes to one another (Fig. 2C).
As with systemic MTP-131 treatment, the restoration of function with eye drop application of MTP-131 was independent of overt changes in the quality of the optical axis (data not shown).
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Keeping with this, no overt change in the general pattern of tyrosine phosphorylation was observed upon TCR stimulation, further arguing against a general impairment of TCR signaling in the absence of USP34.
We note that the endocrine secreting CCK+ cells in the mutant duodenum epithelium are present, implying not an overt change in the Gas1 mutant epithelium patterning (supplementary material Fig. S2).
To test the contribution of olfactory input to the establishment of functional asymmetries, we compared visual responses in fish for which the olfactory pits were ablated at 1 dpf, prior to habenular innervation by mitral cells, and, again, no overt change in the asymmetry of visual responses was observed.
This idea was fortified by the findings that the only overt change in the composition of yeast spliceosomal snRNPs in tgs1 ∆ cells was the gain of CBC as a stoichiometric component of the U1 snRNP, by virtue of its binding to the residual mG cap on the U1 snRNA (Schwer et al. 2011).
Additionally, an increase, especially in urinary NGAL levels, in septic patients may be indicative of inflammatory tubular injury without overt changes in GFR, the so-called subclinical kidney injury that is very difficult to detect and research.
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