Exact(11)
Murdoch's audacious overnight transfer of his newspapers to Wapping, east London, in 1986 proved he hated the trade unions, but what he likes is more difficult to pinpoint.
That is why the Bank of Mexico and America's Federal Reserve are running a programme, called Directo a México, to cut the cost further.Under this scheme, people receive an overnight transfer from an American bank account to a Mexican one.
If you freeze it overnight, transfer it to the refrigerator for at least 1 hour to soften before serving.
Typically, 20 μl of sample was separated on SDS/PAGE (10% gel) before overnight transfer on to nitrocellulose.
Following overnight transfer, Western blots were fixed with 2.5% glutaraldehyde, blocked with 5% milk/TBS-T, and incubated with anti-HA.11 (1:1,500) for 2 hr.
After separation (90 minutes, 150 V), overnight transfer (30 V) to nitrocellulose was performed and then blocking (5% milk PBS/Tween 0.1%, 2 hours, 20°C).
Similar(49)
Excised fat pads were immediately fixed in Zn-formalin overnight, transferred to 70% ethanol, and subsequently paraffin-embedded.
Embryos were harvested, rinsed with PBS, fixed in 4% paraformaldehyde in PBS at 4°C overnight, transferred to 30% sucrose solution, followed by flash freezing, and cutting into 20 µm sections with a cryostat.
High-molecular-weight RNA (10 µg total RNA) were submitted to electrophoresis on a 0.9% agarose-formaldehyde gel [43], and overnight transferred to positively charged Hybond™-N+ membranes (GE Healthcare) by capillary elution with 20× SSC.
Pathologically altered organs, entire reproductive tracts and representative specimens of multiple organs and tissues, including the brain, lung, liver, kidney, spleen, pancreas and intestine were removed at necropsy, fixed in 10% (v/v) neutral buffered formalin (NBF) overnight, transferred to 70% ethanol and paraffin-embedded.
The brains were then removed, fixed overnight, transferred to 30% sucrose, and stored at 4°C.
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